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Genesis provides research laboratories, universities, biotechnology companies, and other institutions with laboratory products and biotechnology supplies across key research applications.
Extenesis Long Taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragments. This specially formulated Taq was shown to amplifiy long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex templates, such as GC-rich templates.
Extenesis Long taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Extenesis Long Taq in your PCR reactions results in 3-dA overhangs, which can be used in TA cloning.
Applications • PCR amplification of DNA fragments as long as 5 kb • DNA labeling • DNA sequencing • PCR for cloning
Unit Definition One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP’s into an acid-insoluble form in 30 minutes at 70°C using herring sperm DNA as substrate.
• High fidelity: three times the fidelity of Taq DNA Polymerase. • Longer fragments: amplifies templates as long as 40kb. • Amplification of complex templates (GC rich or repetitive sequence). • Generates 3'-dA and blunt end PCR products.
Note:
• 10x Extenesis Long PCR BufferⅠ is our classical Extenesis Long Taq DNA Polymerase buffer and is good for long templates especially above 10kb.
• 10xLong PCR Buffer Ⅱ is a special optimized buffer. It is for better fidelity but not good at long templates above 10kb.
• Users should choose suitable buffer for different templates.
