Building Biotechnology Capabilities Across Africa Delivering laboratory products, custom molecular biology solutions, and biomanufacturing expertise to accelerate scientific discovery.
Genesis provides research laboratories, universities, biotechnology companies, and other institutions with laboratory products and biotechnology supplies across key research applications.
Note This system contains sufficient reagents suitable for 100 amplification reactions in a 20 μl PCR system.
Storage This reagent can be stored at 4°C for 2 months and protected from light. For longer storage, this reagent should be kept at -20°C and protected from light.
Description
Genesis 2x SYBR® Green qPCR Mix (High Rox+) is designed for high-performance, high-throughput, real-time PCR. The kit contains a Taq DNA polymerase engineered through a process of molecular evolution. The result is a unique enzyme, specifically designed for qPCR using SYBR® Green I dye chemistry.
2x SYBR® Green qPCR Mix (High Rox+) is a convenient premix of the components (except primers, template, and water) necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green dye binds to double-stranded (ds) DNA, thus providing a fluorescent signal that reflects the amount of dsDNA product generated during PCR. This reagent is used in amplification and detection of DNA in qPCR on ABI real-time instruments that support normalization with Rox reference dye at a final concentration of 50 nM.
Features
• This reagent can be used in ABI Real-time systems that require low concentration of Rox reference
dye.
• Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible amplification.
Composition of the SYBR Green qPCR Mix 100 mM KCl , 5 mM MgCl2, 400 μM dNTPs, 0.1 U/μl Hot Start Taq DNA Polymerase, 1x SYBR® Green, 0.2% Rox reference dye and other optimized buffer components.
Detection • This reagent can be used in general detection devices, such as: LineGene (Bioer Technology co., ltd.) • This reagent can also be used in detection equipment using glass capillaries or passive reference, such as: LightCycler (Roche Molecular Systems) ABI PRISM® 7000, 7700, and 7900 (Applied Biosystems) Note: The passive reference mode of detectors should be set at "ROX”.
