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Description Taq DNA Polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5' → 3' DNA polymerase and a 5' → 3' exonuclease activity but lacks a 3' → 5' exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.
Applications Standard PCR DNA labeling DNA sequencing Numerous applications for which a high-quality, thermostable DNA polymerase is required
Unit Definition One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using herring sperm DNA as a substrate.
