Building Biotechnology Capabilities Across Africa Delivering laboratory products, custom molecular biology solutions, and biomanufacturing expertise to accelerate scientific discovery.
Genesis provides research laboratories, universities, biotechnology companies, and other institutions with laboratory products and biotechnology supplies across key research applications.
Quick Tissue/Culture Cells DNA Extraction Kit 50 Preps
SKU
GN1141
$160.00
1
Product Details
Description
Our Tissue/Culture Cells Genomic DNA Extraction Kit provides a simple and rapid method for high quality genomic DNA purification from mammalian tissues (either fresh or frozen at -70°C until use) and culture cells. The Tissue/Culture Cells Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-35 µg from 10 mg of tissue or 1 x 106-107 cultured cells. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.
Features Efficient: 3-35 µg of genomic DNA from 10 mg of tissue or 106-107 cultured cells.
Fast: Procedure takes only 30 min.
Universal: Purifies genomic DNA from various sources.
Safe: No phenol/chloroform extraction step.
High purity: Purified DNA is ready for downstream applications such as PCR and restriction enzyme digestion.
Downstream Applications The purified DNA is free from contaminants and enzyme inhibitors, typically has A260/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:
