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Genesis provides research laboratories, universities, biotechnology companies, and other institutions with laboratory products and biotechnology supplies across key research applications.
Note This system contains sufficient reagents suitable for 100 amplification reactions.
Storage This reagent can be stored at 4°C for 2 months protected from light. For longer storage, this reagent should be kept at -20°C and protected from light.
Description
This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assays with SYBR® Green I
Features • This reagent can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.). • This reagent can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems. • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible amplification.
Composition of the SYBR Green qPCR Mix 100 mM KCl , 4 mM MgCl2, 400 μM dNTPs, 0.1 U/μl Taq DNA Polymerase, 1x SYBR® Green and other optimized buffer components
Detection • This reagent can be used in general detection devices, such as: LineGene (Bioer Technology Co., ltd.) • This reagent can also be used in detection equipment using glass capillaries or passive reference, such as: LightCycler (Roche Molecular Systems) ABI PRISM® 7000, 7700, and 7900 (Applied Biosystems) Note: The passive reference mode of detectors should be set at "ROX”.
