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Hi-Fidelity DNA Polymerase Mg+ 250 U

SKU GH1031
$35.00
On Sale
was $50.00 Save 30%
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Product Details
Hi-Fidelity DNA Polymerase (2.5 U/µl

100 µl
10X PCR Buffer (Mg+2 Plus)

1.25 ml
6X Loading Buffer 1 ml

Description

Hi-Fidelity DNA Polymerase, a mixture of Taq and Pfu polymerase, blends the processivity of Taq with the high fidelity of Pfu. Therefore, this specially formulated Hi-Fidelity DNA Polymerase allows amplification with high fidelity of longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex templates, such as GC-rich templates, and it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3´-dA overhangs, which can be used in TA clone.

Features

High fidelity: Its fidelity is four times higher than ordinary Taq polymerase.
High yields: Suitable for amplifying large fragments; suitable for complex templates, which are rich in GC or repeat sequences.

Applications
Long PCR with high fidelity
Amplification of complex templates

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

Functionally tested in PCR

Definition of Activity Unit
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM TrisCl ( pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT,0.1% NP-40 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol


10X PCR Buffer

100mM Tris-HCl(PH 8.8), 500mMKCl, 1%Triton-X-100, 16mM MgCl2


Store all components at –20°C

Product Number: GH1031

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Hi-Fidelity DNA Polymerase Mg+ 250 U

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