Building Biotechnology Capabilities Across Africa Delivering laboratory products, custom molecular biology solutions, and biomanufacturing expertise to accelerate scientific discovery.
Genesis provides research laboratories, universities, biotechnology companies, and other institutions with laboratory products and biotechnology supplies across key research applications.
Our Quick Blood Genomic DNA Purification Kit provides a simple and rapid method for high quality genomic DNA purification from whole blood. Compared to the traditional blood genomic DNA extraction kit, the quick system provides a more simple method since it doesn’t have to remove erythrocytes from whole blood. The Quick Blood Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 5 µg from 200 μl of whole blood. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.
Features
Fast: Procedure takes only 30 min. Simple: No need to remove erythrocytes from whole blood Efficient: 5 µg from 200 μl of whole blood. Safe: No phenol extraction step. High purity: Purified DNA is ready for downstream application such as PCR and restriction digestion.
Downstream Applications
Purified DNA is free from contaminants and enzyme inhibitors, and typically has A260/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:
Restriction digestion
PCR
Labeling
Library construction
Important Notes
• Prior to the initial use of the kit, dilute the Wash Buffer(PE) with ethanol (96-100%)
Storage
Store Protein K at -20℃, other reagents can be stored at room temperature for up to 1 year. Any precipitate in the Solution DS and Solution QS can be re-dissolved by incubating at 37°C before use.
