Building Biotechnology Capabilities Across Africa Delivering laboratory products, custom molecular biology solutions, and biomanufacturing expertise to accelerate scientific discovery.
Genesis provides research laboratories, universities, biotechnology companies, and other institutions with laboratory products and biotechnology supplies across key research applications.
Our M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Our M-MLV Reverse Transcriptase has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Source E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Applications Generation of first strand cDNA for use in: -PCR, see Protocol for First-strand cDNA Synthesis; -real-time PCR; -second strand cDNA synthesis; DNA labeling; Analysis of RNA by primer extension.
Quality Control The absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.
Functionally tested in first strand cDNA synthesis.
