Our 20bp DNA Ladder is ideal for determining the size of double-stranded DNA from 60 to 300 base pairs. The ladder consists of 13 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.
Recommended Loading
2-5 µl/Lane
Concentration Typical Bands 100 ng/5µl
Other Bands 40 ng/5µl
Advised Electrophoresis Conditions
1 µl/Lane, 20 cm ,10 %Polyacrylamide Gel, 1×TBE, 8V/cm, 3 h.
