Our 2X Fast Taq Mix For Blood is a premixed, ready-to-use solution containing Fast Taq DNA Polymerase, dNTPs, and all other PCR components, except the DNA template and primers. Fast Taq Mix For Blood is specific for whole blood amplification. It contributes to fast, specific, sensitive and reproducible PCR by reducing the risk of pipetting errors, miscalculation, and contamination. The 2X Fast Mix (2X) For Blood can be used with conventional PCR machines. This product cuts off the complicated and time-wasting process of DNA extraction and minimizes cross contamination in the reaction.
Fast Taq DNA Polymerase is the latest generation of Taq-based DNA polymerase. It possesses high amplification efficiency like Taq polymerase, and fast elongation ability like KOD polymerase, and can be use in various kinds of PCR. The Fast PCR Buffer is designed for Fast Taq DNA polymerase, and can be used in fast amplification reactions. Fast Taq DNA polymerase has an elongation rate 2x higher than regular Taq DNA polymerase, and can shorten the amplification time by one-half. It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity, which results in a 3'-dA overhangs.
Features • Convenient: Direct whole blood PCR amplification. • High yields of PCR products with minimal optimization • Fast: saves time due to reduced number of pipetting steps • Reproducible: lower contamination and pipetting error risk • Higher sensitivity and faster compared to conventional Taq DNA polymerase
Applications
• Amplification for Whole Blood
• High throughput PCR • Long and complex template PCR
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.Contents
