Note This system contains sufficient reagents suitable for 40 amplification reactions of 50 μl each.
Description 2X Our Hi-Fidelity PCR Master Mix (“Plus Mix”) is a premixed, ready-to-use solution containing Our Hi-Fidelity DNA Polymerase, dNTPs, MgCl2 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Plus Mix contributes to a highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding enhancer.
Our Hi-Fidelity polymerase, a mixture of taq and pfu polymerase, blends the processivity of taq with the high fidelity of pfu. Therefore, this specially formulated Taq plus allow amplification of the higer fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex templates, such as GC-rich template, and it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using the Hi-Fidelity polymerase results 3´-dA overhangs in PCR products, which can be used in TA clone.
Features Convenient : only primers and template DNA are added when preparing the PCR High efficiency: saves your time by simplifying the process Reproducible: reduces the risk of pipetting errors, miscalculation and contamination
Applications Long PCR with high fidelity Highly reproducible PCR for complex templates High throughput PCR for complex templates Generates PCR products for TA cloning
Unit Definition One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using herring sperm DNA as substrate.