2X Pfu Mix 1 ml

Contents:

40 reactions of 50 µl each

Description:
Our 2X Pfu Mix is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO₄and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR solution, only primers and template DNA are added. Our Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding intensifier and optimizer.

Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerases. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

Applications
High fidelity PCR
Highly reproducible PCR
PCR cloning
Site-directed mutagenesis

Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using herring sperm DNA as the substrate.

2×Pfu Mix

0.15U/µl Pfu DNA polymerase, 2x Pfu Buffer, 0.4mM dNTPs, 4mM MgSO4, 0.02% bromophenol blue.

Store at -20°C

Product Number: G2021