Note The system contains sufficient reagents suitable for 40 amplification reactions of 50 μl each.
Description Our Taq PCR Mix (2X) is an optimized ready-to-use PCR mixture of Recombinant Taq DNA Polymerase, PCR Buffer, MgCl2 and dNTPs. PCR Mix (2X) contains all components for PCR, except DNA template and primers.
Applications Standard PCR DNA labeling DNA sequencing Numerous applications for which a high-quality thermostable DNA polymerase is required
Unit Definition One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
2×Taq Mix
0.25U/ul Taq DNA polymerase, 2X PCR buffer, 0.4mM dNTPs, 3.2 mM MgCl2, 0.02% bromophenol blue.
