Description Our 500 bp Ladder is ideal for determining the size of double-stranded DNA from 500 to 5,000 base pairs. The ladder consists of 10 linear double-stranded fragments. The 2,000 bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 2,000 bp are 40 ng. The 2,000 bp fragment is 100 ng. This ladder is pre-mixed with loading dye and is ready to use.
Recommended Loading 2-5 µl/Lane
Concentration Typical Bands 100 ng/5µl Other Bands 40 ng/5µl
Advised Electrophoresis Conditions 8 cm, 1% Agarose Gel, 1×TAE, 7 V/cm, 45 min
