LE-Agarose 1200 is a low EEO, high gel strength agarose for analysis of nucleic acid molecules. Typical applications include electrophoretic separation of DNA and RNA, Southern & Northern blotting, and also lmmunodiffuson and Ouchterlony of proteins.
Analytical Specifications
EEO (-m r)
0.1-0.15
Water Content
≤10%
Sulfate (SO4)
0.15-0.2%
Gel Strength (1% gel)
≥1200 g/cm2
Gelling Point (1.5% gel)
33±1.5oC
Melting Point (1.5% gel)
87±1.5oC
Concentrations for DNA separation The appropriate agarose concentration depends on the size of the DNA fragments to be separated, the buffer used, and the applied voltage. With IX TAE buffer, the suggested concentrations for DNA sizes in base pairs (bp) follows :
