Extenesis Long Taq DNA Polymerase 1000 U

Description

Extenesis Long Taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragments. This specially formulated Taq was shown to amplifiy long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex templates, such as GC-rich templates.

Extenesis Long taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Extenesis Long Taq in your PCR reactions results in 3-dA overhangs, which can be used in TA cloning.

Applications
• PCR amplification of DNA fragments as long as 5 kb
• DNA labeling
• DNA sequencing
• PCR for cloning

Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP’s into an acid-insoluble form in 30 minutes at 70°C using herring sperm DNA as substrate.

Storage Buffer
20mM TrisCl ( pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT，0.1% NP-40, 0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol

10X Extenesis Long Taq Buffer Ⅰ with Mg⁺²

500mM Tris-HCl pH 8.8，160mM (NH4)2SO4 ，25mM MgCl2 ，1% Triton X-100

10X Extenesis Long Taq Buffer Ⅱ with Mg⁺²

200mM Tris-HCl PH8.8，100mM KCl，100mM (NH4)2SO4，16mM MgSO4，1% Tritonx-100

Features

• High fidelity: three times the fidelity of Taq DNA Polymerase.
• Longer fragments: amplifies templates as long as 40kb.
• Amplification of complex templates (GC rich or repetitive sequence).
• Generates 3'-dA and blunt end PCR products.

Note:

• 10x Extenesis Long PCR BufferⅠ is our classical Extenesis Long Taq DNA Polymerase buffer and is good for long templates especially above 10kb.

• 10xLong PCR Buffer Ⅱ is a special optimized buffer. It is for better fidelity but not good at long templates above 10kb.

• Users should choose suitable buffer for different templates.

Product Number: GE1063