Note This system contains sufficient reagents suitable for 100 amplification reactions.
Storage This reagent can be stored at 4°C for 2 months protected from light. For longer storage, this reagent should be kept at -20°C and protected from light.
Description
This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assays with SYBR® Green I
Features • This reagent can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.). • This reagent can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems. • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible amplification.
Composition of the SYBR Green qPCR Mix 100 mM KCl , 4 mM MgCl2, 400 μM dNTPs, 0.1 U/μl Taq DNA Polymerase, 1x SYBR® Green and other optimized buffer components
Detection • This reagent can be used in general detection devices, such as: LineGene (Bioer Technology Co., ltd.) • This reagent can also be used in detection equipment using glass capillaries or passive reference, such as: LightCycler (Roche Molecular Systems) ABI PRISM® 7000, 7700, and 7900 (Applied Biosystems) Note: The passive reference mode of detectors should be set at "ROX”.
