Note This system contains sufficient reagents suitable for 100 amplification reactions in a 20 μl PCR system.
Storage This reagent can be stored at 4°C for 2 months and protected from light. For longer storage, this reagent should be kept at -20°C and protected from light.
Description
Genesis 2x SYBR® Green qPCR Mix (Low Rox+) is designed for high-performance, high-throughput, real-time PCR. The kit contains a Taq DNA polymerase engineered through a process of molecular evolution. The result is a unique enzyme, specifically designed for qPCR using SYBR® Green I dye chemistry.
2x SYBR® Green qPCR Mix (Low Rox+) is a convenient premix of the components (except primers, template, and water) necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green dye binds to double-stranded (ds) DNA, thus providing a fluorescent signal that reflects the amount of dsDNA product generated during PCR. This reagent is used in amplification and detection of DNA in qPCR on ABI real-time instruments that support normalization with Rox reference dye at a final concentration of 50 nM.
Features
• This reagent can be used in ABI Real-time systems that require low concentration of Rox reference
dye.
• Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible amplification.
Composition of the SYBR Green qPCR Mix 100 mM KCl , 5 mM MgCl2, 400 μM dNTPs, 0.1 U/μl Hot Start Taq DNA Polymerase, 1x SYBR® Green, 0.2% Rox reference dye and other optimized buffer components.
Detection • This reagent can be used in general detection devices, such as: LineGene (Bioer Technology co., ltd.) • This reagent can also be used in detection equipment using glass capillaries or passive reference, such as: LightCycler (Roche Molecular Systems) ABI PRISM® 7000, 7700, and 7900 (Applied Biosystems) Note: The passive reference mode of detectors should be set at“ROX”.
