Hi-Fidelity DNA Polymerase, a mixture of Taq and Pfu polymerase, blends the processivity of Taq with the high fidelity of Pfu. Therefore, this specially formulated Hi-Fidelity DNA Polymerase allows amplification with high fidelity of longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex templates, such as GC-rich templates, and it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3´-dA overhangs, which can be used in TA clone.
Features
High fidelity: Its fidelity is four times higher than ordinary Taq polymerase. High yields: Suitable for amplifying large fragments; suitable for complex templates, which are rich in GC or repeat sequences.
Applications Long PCR with high fidelity Amplification of complex templates
Quality Control The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
Functionally tested in PCR
Definition of Activity Unit One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
