Description Our Plasmid Maxiprep Kit is designed for rapid and cost-effective large-scale preparation of high quality plasmid DNA from recombinant E.coli cultures. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. Each spin column can recover 400-1,000 μg of plasmid DNA from 100 ml overnight bacterial culture. The kit can be successfully used for efficient purification of any size plasmids and cosmids. The actual plasmid yield and optimal culture volume depend on the plasmid copy number and medium used for cultivation.
The method is based on a silica-membrane spin column; silica particles bind DNA selectively at high salt concentration and low pH, while protein and other contaminants are washed away. The pure DNA is eluted with TE buffer or water. This method requires few manipulations, and is both faster and easier to perform than other organic-based extraction methods. The purified DNA is suitable for all common molecular biology procedures, including restriction digestion, cloning, sequencing, etc.
Features High yield : A 100 ml suspension yields 400-1,000 μg of plasmid DNA high copy numbers. High purity : A260/A280=1.7-1.9. with no chloroform and phenol. Fast : Purification takes only 40 min.
Storage RNase A: store at -20℃. Before starting, please add RNase A to Solution I, mix well and store at 4℃. Other reagents can be store at room temperature. If precipitate forms in the buffers during storage, it should be redissolved by incubating the buffers at 37℃ before use.
