Our Genesis Hot Start Taq DNA polymerase is a hot-start polymerase with a chemical modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length and speed can reach up to 5 kb (simple template) and 0.5 kb/min, respectively. Hot Start Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The products of Taq plus have dA overhangs at 3’-end.
Genesis Hot Start Taq DNA polymerase has zero animal source pollution, since it is produced with advanced chemical modification. It is also much more stable than an antibody-modified hot-start polymerase. Its efficiency is higher than most chemical-modified polymerases and the initial-denaturation time can be reduced to 3 minutes.
Genesis Hot Start Taq DNA polymerase is an innovative and useful product.
Unit Definition
One enzyme unit (U) refers to the amount of enzyme needed for processing 10 nmol of nucleotides when using activated salmon sperm DNA as template/primer, at 72 ℃ in 30 minutes
Quality control
The absence of endonuclease or exonuclease is confirmed by appropriate quality tests. PCR detects no host residual DNA and it can effective amplification of a single-copy gene in a human genome. There is no significant change in the amplification activity after one week at room temperature.
Storage Buffer
200 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Tween 20, 50% Glycerol
0.5% Triton X -100
10X Hot Start Buffer with Mg2+
50 mM KCl, 100 mM Tris-HCl, 200 mM NH4Cl, 20 mM MgCl2
