Our M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Our M-MLV Reverse Transcriptase has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Source E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Applications Generation of first strand cDNA for use in: -PCR, see Protocol for First-strand cDNA Synthesis; -real-time PCR; -second strand cDNA synthesis; DNA labeling; Analysis of RNA by primer extension.
Quality Control The absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.
Functionally tested in first strand cDNA synthesis.
